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test results step 8: After incubating 24 hours, remove the BI vials from the incubator. o Take a third spore ampule (labeled as control ampule) that was not run in the load. o Take both ampoules from the loads that were autoclaved, gently squeeze the plastic crusher to break the glass ampule and place those in the incubator. Growth of surviving spores has been documented as little as 2.5 hours. Use an adjusted microbiological incubator to a temperature for 131F 140F (55C to 60C). You can change your choices at any time by visiting Cookie Preferences, as described in the Cookie Notice. Incubate at the preset temperature of 55-60 C for 24 hours checking for spore growth at regular intervals (i.e. Click ‘Customise Cookies’ to decline these cookies, make more detailed choices, or learn more.

The Third Wave Grow Kit will more than pay for itself in just one harvest. That means a single harvest can yield 280-1,080 worth of your very own mushrooms. With the Third Wave Grow Kit, the average harvest ranges from 1-4 ounces (28-108 grams). Third parties use cookies for their purposes of displaying and measuring personalised ads, generating audience insights, and developing and improving products. A single gram of many popular mushroom species can cost as much as 10 per gram.

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